Chiral DNA packaging in DNA-cationic liposome assemblies

Attomolar DNA detection with chiral nanorod assemblies

Nanoscale plasmonic assemblies display exceptionally strong chiral optical activity. So far, their structural design was primarily driven by challenges related to metamaterials whose practical applications are remote. Here we demonstrate that gold nanorods assembled by the polymerase chain reaction into DNA-bridged chiral systems have promising analytical applications. The chiroplasmonic activi...

preparation and in vitro transfection of modified cationic liposome-linear polyethyleneimine-dna nanocomplexes for gene delivery

Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes.

Cationic liposomes complexed with DNA (CL-DNA) are promising synthetically based nonviral carriers of DNA vectors for gene therapy. The solution structure of CL-DNA complexes was probed on length scales from subnanometer to micrometer by synchrotron x-ray diffraction and optical microscopy. The addition of either linear lambda-phage or plasmid DNA to CLs resulted in an unexpected topological tr...

Two-Dimensional Smectic Ordering of Linear DNA Chains in Self-Assembled DNA-Cationic Liposome Mixtures

We report a synchrotron x-ray scattering study of linear DNA chains and cationic liposome mixtures which spontaneously self-assemble into a coupled two-dimensional (2D) smectic phase of DNA chains imbedded between lipid bilayers of a 3D smectic phase. The DNA peak is quantitatively described by anisotropic exponentially decaying chain-chain correlations. The measured interchain compressibility ...

An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery.

A two-dimensional columnar phase in mixtures of DNA complexed with cationic liposomes has been found in the lipid composition regime known to be significantly more efficient at transfecting mammalian cells in culture compared to the lamellar (LalphaC) structure of cationic liposome-DNA complexes. The structure, derived from synchrotron x-ray diffraction, consists of DNA coated by cationic lipid...